Evaluation of Anti-Infectious Activity of Bioconjugates Based on Quantum Dots CdTe / Cd MPA 710 and Levofloxacin against Staphylococcal Corneal Infection. Experimental Research

Abstract

Infectious keratitis is one of the leading causes of persistent decline in visual function and monocular blindness in both developed and developing countries. The combination of factors in the prevalence of eye infectious and inflammatory diseases, antibiotic resistance, and internal mutations of the pathogens themselves exacerbate the need to search for highly effective alternatives in the fight against eye infectious diseases. Of particular interest are the prospects for the use of fluorescent semiconductor nanocrystals, called quantum dots, in the treatment of resistant infectious inflammatory diseases. The objective of the study was to assess the anti-infectious activity of bioconjugates based on CdTe / Cd MPA 710 quantum dots and levofloxacin against staphylococcal corneal infection using the example induced infectious keratitis in laboratory animals. As the object of the study, 6 male (6 eyes) New Zealand rabbits were studied, which underwent induction of bacterial keratitis by introducing a hospital strain of S. Aureus into the structure of the cornea. The following were used as antimicrobial agents: a solution of levofloxacin 5 ml for epibulbar use and a bioconjugate based on CT CdTe / Cd MPA 710 and levofloxacin.