Abstract

Phnom Kulen National Park, in north-western Cambodia, has huge richness in biodiversity and medicinal value. One hundred and ninety-five (195) medicinal plant species were collected from the national park to examine allelopathic potentials by using the sandwich method, a specific bioassay for the evaluation of leachates from plants. The study found 58 out of 195 medicinal plant species showed significant inhibitory effects on lettuce radicle elongation as evaluated by standard deviation variance based on the normal distribution. Three species including Iris pallida (4% of control), Parabarium micranthum (7.5% of control), and Peliosanthes teta (8.2% of control) showed strong inhibition of lettuce radicle elongation less than 10% of the control. The results presented could present as a benchmark for isolation and identification of allelochemicals among medicinal plants used in Cambodia.

Highlights

  • Plant species in the natural diversity have been used by humans to treat numerous diseases worldwide

  • The term allelopathy was introduced by Molisch in 1937, referring to a phenomenon observed in many plants that influence the physiological process of neighbouring plants and or organisms, interacting through secondary

  • The elongation percentages of radicle and hypocotyl of lettuce seedlings were affected by leachates from was medicinal in therandomized sandwich bioassay

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Summary

Introduction

Plant species in the natural diversity have been used by humans to treat numerous diseases worldwide. Medicinal plants are a significant source of bioactive substances in the development of most drugs [4,5]. Bioactive phytochemical constituents include alkaloids, tannins, flavonoids and some other phenolic compounds present in medicinal plants that produce a definite physiological action effect either on humans, animals, and other plants [6]. Some bioactive compounds contained in medicinal plants including ferulic, coumaric, vanillic, caffeic and chlorogenic acids in medicinal plants were found to possess plant growth inhibitory effect [8,9]. The term allelopathy was introduced by Molisch in 1937, referring to a phenomenon observed in many plants that influence the physiological process of neighbouring plants and or organisms, interacting through secondary

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