Abstract
Background: The pathogenesis of inflammatory bowel disease may be associated with the disruption in interactions between the immune system and gut flora. Epigenetic mechanisms especially, DNA methylation appear to be significant regarding the interaction between the environment and genome. ABCB1 is the encoding gene for multi-drug resistance protein 1 (MDR1) (P-glycoprotein), which is an important transmembrane protein responsible for the efflux of cellular molecules from the intestinal wall to the lumen Method: In this study, we compared the methylation status of the promoter of ABCB1 in rectal mucosa of patients with ulcerative colitis (UC) and healthy controls by using the bisulfite conversion system and realtime quantitative multiplex methylation-specific PCR (QM-MSP). Results: We demonstrated that the mucosal specimen of 26 UC patients had significantly higher levels of promoter methylation in comparison to 26 controls. Conclusion: As the first investigation of Iranian patients with UC, we showed that patients had higher levels of ABCB1 promoter methylation in their inflammatory rectal mucosa compared to controls. However, this altered state of methylation did not associate with the characteristics of the patients such as age and sex. Our findings are a basis for further studies on concurrent assessment of promoter methylation and expression of ABCB1 in UC.
Published Version
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