Evaluation of a biosurfactant producing bacterial strain Pseudomonas sp. ISTPY2 for efficient pyrene degradation and landfill soil bioremediation through soil microcosm and proteomic studies

Abstract

Landfill soil pollution is a huge cause of concern particularly in the developing countries like India where unsegregated wastes are disposed off in the open dumping sites, posing a high health risk to workers and rag pickers. This necessitates an efficient strategy for landfill soil reclamation. The present study evaluates the efficiency of a biosurfactant producing strain Pseudomonas sp. ISTPY2 isolated from landfill soil for pyrene degradation and landfill soil remediation in a soil microcosm. The metabolites were identified using GC–MS and an increase in the number of aliphatic compounds was observed during the process with phthalic acid and alkanes as major metabolites. Proteomic study was performed to study the enzymes involved in pyrene degradation and it was found that various xenobiotic degrading proteins such as phthalate 4,5-dioxygenase, aldehyde dehydrogenase, F420-dependent oxidoreductase and others were induced in response to pyrene stress and played a role in its efficient biodegradation.