Abstract
The present study demonstrates pyrene degradation by a pshychrophilic bacterial strain, Shewanella sp. ISTPL2 isolated from Pangong lake, Ladakh, India. The ISTPL2 strain showed highest specific growth rate at 500 ppm with 93% extent of degradation. Pyrene metabolic pathway was confirmed from metabolites such as 4,5-dihydroxypyrene, 2-carboxybenzalpyruvate, phthalic acid and salicylic acid obtained from pyrene degradation. Soil microcosm was set up with different concentration of pyrene and investigated using GC–MS to uncover the arrangement of intermediary metabolites. The results indicated that 75.11%, 95.39%, 94.32% and 93.11% of pyrene was degraded within 90 days in the soil spiked with 50, 350, 500 and 1000 ppm pyrene respectively. Strain ISTPL2 is a potent candidate for pyrene degradation as it can survive under high pyrene load.
Published Version
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