Abstract
Typical steroids in ointment were studied by thin layer chromatography (TLC) using a cyanopropyl-chemically bonded stationary phase, prepared from pre-coated silica gel plate treated with toluene solution containing 3-cyanopropyltrichlorosilane (CN). TLC plate with CN was applied to the evaluation of typical steroids in ointment samples. The CN treated plate was developed in a chromatographic chamber at room temperature with chloroformmethanol (95: 5 v/v) to a distance of 8cm from the origin.After drying, the plate was sprayed with 10% sulfuric acid solution, and heated. Steroid spots on the chromatogram were measured with a TLC densitometer equipped with a dual-wave length TLC scanner at λEX 380nm, λEM 550nm.
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