Abstract
The use of a suitable biological material, represented by the normal cell culture cell line RM, consisting of monkey kidney cells or of suitable experimental models allow the investigation of the reactivity of the cell protein synthesis process (illustrated by the total protein content of the cell cultures aged 72 hours, after a 48-hour treatment), cell proliferation (expressed by the evolution of the total cell number during cell culture development), cell viability (characterized by the dynamics of the dead / living cells ratio), cell apoptosis and cell culture development. Alteration of these processes reflects the cytotoxic impact of some products on protein synthesis, mitosis, cell apoptosis and cell viability.
Highlights
The use of a suitable biological material, represented by the normal cell culture cell line RM, consisting of monkey kidney cells or of suitable experimental models allow the investigation of the reactivity of the cell protein synthesis process, cell proliferation, cell viability, cell apoptosis and cell culture development
In order to obtain direct indications regarding the interaction of the agents investigated with the process of cell mitosis, with apoptosis and cell viability, we developed a new experimental model compatible with their evaluation during the evolution of the different cell culture variants of RM, in which the modern flow cytometry technique was used
We will present the results of our investigations on the in vitro reactivity of monkey kidney cells, by the action of samples from Titanium, Nitinol, Verasoft (Cr-Ni), Superpont, biochemically characterized as ionic diffusers, obtained from different dental materials in artificial incubation saliva
Summary
A number of 5 preparations were included in the cytotoxicity testing circuit, coded as follows: SA - (artificial saliva, incubation environment of various dental materials); SA - Titan (ionically diffused in artificial saliva); SA - Verasoft (ionic diffused in artificial saliva); SA - Superpont (ionic diffused in artificial saliva); SA - Nitinol (ionic diffused in artificial saliva). The biological material used in the in vitro test experiments of the cytotoxic impact of the eluates was represented by normal, non-contaminated kidney cell cultures (obtained from the monkey kidneys of Cercopithecus aethiops), maintained in 25 cm plates. In order to obtain direct indications regarding the interaction of the agents investigated with the process of cell mitosis, with apoptosis and cell viability, we developed a new experimental model compatible with their evaluation during the evolution of the different cell culture variants of RM, in which the modern flow cytometry technique was used. Investigation of the cytotoxic effect of the samples included in the in vitro screening on normal animal eukaryotic cell cultures required: estimation of the total protein concentration at 72 hours of the control, reference and treated cultures for 48 hours; establishing the impact of the studied biological preparations on the cell proliferation process and appreciating the amplitude of their mito-. There were used for each category of monkey kidney cell culture and five culture tubes, this being the minimum number required for statistical calculation using Student’s t test and histograms
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