Abstract
Chronic renal failure is the progressive loss of function of kidney and patient requires a long treatment in the form of renal replacement therapy. Alanine aminopeptidase activity has been assayed in (70) patient’s sera samples of both sexes, aged (25-70) years. The sample were divided in to two groups, the first group(G1) is of (35) samples before dialysis. The second group(G2) is of (35) samples after dialysis, as well as (30) sample of healthy as control group(C). The result showed a significant increase (p> 0.001) in general in the enzyme Alanine aminopeptidase in the serum of chronic renal failure patients (116.0±3.4) U/L while the efficacy rate is (105.5±3.4) U/L in healthy persons. Goal of the research was partial purified of enzyme from sera patients with chronic renal failure. by using two techniques: The first gel filtration chromatography packed with spadix G50 and giving 3.45% yield and 0.964-fold purification. Two isoenzymes were obtained by using ion exchange chromatography and the purity degree of isoenzymes were 0.687(1.08%) and 2.539(1.04%) fold respectively. Also, the results showed a single band for isoenzymes following steps.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.