Abstract

The influence was investigated of several environmental factors [feed quantity and quality, temperature (6, 11 or 18°C), osmolarity (9 or 580 mOsm/l)) acting simultaneously on the activity of an amino acid arylamidase, alanine aminopeptidase (AAP; E.C. 3.4.1.-) in two midgut sections of rainbow trout. AAP activity in the proximal section, which accounts for 95% of the total activity in the midgut, was found to be affected by interactions of the ambient temperature and the feed quality. The activity increased considerably on a high protein, low fat diet after adaptation to 6°C. The AAP activity varied inversely to the dietary protein content, but only if the trout were kept at temperatures of 11 and 18°C. There was a negative non-linear correlation between AAP activity and body weight of trout weighing 20–60 g.

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