Abstract
In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei, RUT C30 and a novel strain, Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2 and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non- supplemented in-house enzymes preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU, resulted in 68% higher glucose yield using wet exploded loblolly pine (WELP) at a 20% DM concetration. The highest saccharifcation yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose and mannose yields, 85%, 92% and 86%, respectively were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.
Highlights
Breast cancer is the most common of all cancers and is the leading cause of cancer deaths in women worldwide, accounting for >1.6% of deaths and case fatality rates are highest in lowresource countries
The activity of Punica granatum was good against cancer cell line .from this we concluded that pomegranate fruit peel extract (PPE) shows a better cancerous activity against the cancer as compare to PSE
The development and refinement of risk prediction models provide an epidemiologic basis for counseling women with a family history that does not appear related to a dominant susceptibility gene
Summary
Breast cancer is the most common of all cancers and is the leading cause of cancer deaths in women worldwide, accounting for >1.6% of deaths and case fatality rates are highest in lowresource countries (www.tmc.gov.in). Conclusion: The activity of Punica granatum was good against cancer cell line .from this we concluded that PPE shows a better cancerous activity against the cancer as compare to PSE. On Thursday, January 2, 1997, Dr Herbert Soule, the scientist who developed the MCF-7 breast cancer cell line, died.
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