Abstract
In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei RUT-C30 and a novel strain Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet-exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2, and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non-supplemented in-house enzyme preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU resulted in 68 % higher glucose yield using wet-exploded loblolly pine (WELP) at a 20 % DM concentration. Highest saccharification yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose, and mannose yields, 85 %, 92 %, and 86 %, respectively, were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
