Evaluating the efficiency of Enzyme-enriched Enzymesporine probiotic feed additive and its impact on the productive properties of pigs in the fattening process

Abstract

The microencapsulation method developed for the Enzyme-enriched Enzymesporine probiotic additive (crystalline trypsin) belongs to the sphere of biotechnology and veterinary medicine. The method is used to produce an additive with a high bioaccessibility value. It maintains the viability of the probiotic microorganisms without the loss of the primary CFU value of 5.5*109 by changing the precipitator (acetone) concentration to 40%. The results, acquired in the in vitro simulation of the digestive process in the stomach, demonstrated the acid-resistance of the microencapsulated additive exposed to hydrochloric acid and pepsin for 120 minutes under pH from 1.5 to 5.5. The introduction of crystalline trypsin at the amount of 5% of the active pharmaceutical ingredient mass caused qualitative improvement in the digestive activity in the pigs’ intestines. The experiments carried out with the pigs of Genesus genetics provided reliable results of the live weight increment and average daily gain within the period from the 49th to 148th days of life to prove the advantage of the encapsulated Enzyme-enriched Enzymesporine probiotic additive compared to the non-encapsulated analogues or the analogues without enzyme.