Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats

Abstract

Objective: In the present paper, it was purposed to examine the late impacts of bone tissue recoveries in combination with synthetic grafts and Ankaferd Blood Stopper (ABS), which are effective in bone wound healing in diabetic rats.
 Methods: A total of 64 Wistar albino male rats with diabetes were studied. A bone deformity was generated in the calvarium of diabetic rats. These diabetic rats are divided into 4 different groups. Only saline was applied to the bone defect in group 1, Beta-tricalcium phosphate (β-TCP) graft was administered to Group 2, ABS solution to Group 3, (β-TCP+ABS) were administered to group 4. Eight rats from each group were sacrificed on the 28th day and another 8 rats on the 56th day. Immunohistochemical, histopathological, and Dual Energy X-ray Absorptiometry (DEXA) analyzes of the obtained samples were made.
 Results: In histopathological measurements, osteoblastic activity and bone regeneration were considerably higher in the group treated with group 2 and group 4 when compared to the control group on the 28th and 56th day (p˂ .05). Western blotting findings showed that the osteopontin (OP) and osteonectin (ON) expression at 28th day increased dramatically in the treated with group 4. DEXA analyzes revealed that BMC values in groups treated with group 2, group 3 and group 4 on 28th day were considerably higher than the control group (p˂ .05).
 Conclusion: We can believe and conclude that ABS in combination with a β-TCP bone graft will produce more successful outcomes on wound
 healing and formation of new bone in diabetic rats.