Evaluating the bio-application of biomacromolecule of lignin-carbohydrate complexes (LCC) from wheat straw in bone metabolism via ROS scavenging

Abstract

Lignin-carbohydrate complexes (LCC) arebiomacromolecules that can be obtained from different biomass. Even some works have shown the LCC can efficiently scavenge the intracellular and endogenous reactive oxygen species (ROS), while little work has been carried out to investigate the potential application of LCC for ROS-related treatment in biological filed, especially for the treatment of periprosthetic osteolysis in vivo. In this work, Lignin-rich (LCC-A) and carbohydrate-rich (LCC-B) fractions in wheat straw are isolated and used as the ROS scavenger to promote osteoblast differentiation and inhibit osteoclast differentiation. The chemical composition and structures are characterized by high performance anion exchange chromatography (HPAEC) and nuclear magnetic resonance (NMR) technologies (quantitative 13C NMR and 2D-HSQC NMR), respectively. The results showed LCC-A possesses higher in vitro ROS-scavenging ability than LCC-B (89.8% vs 57.8%) and to inhibit osteoclast differentiation, whereas LCC-B more significantly activates cellular antioxidant activities via the KEAP1-NRF2-ARE pathway (218.5% vs 438.0% in the level of HO-1), thus promoting osteoblast differentiation in an inflammatory environment. Moreover, the therapeutic administration of LCC-A and LCC-B for Ti-particle-induced osteolytic murine calvariae showed both of them positively regulate and restore the bone metabolism, while preventing calvaria impairment. Hence, LCC from wheat straw exhibits efficient bone protective effects, suggesting it may be used as the promising ROS scavenger for clinical treatment of periprosthetic osteolysis.