Evaluating Non-IgE-mediated Allergens’ Immunoreactivity in Patients with “Intrinsic” Persistent Rhinitis with Help of the Leukocyte Adherence Inhibition Test

Abstract

Background: The non-IgE-mediated immunoreactivities against common allergens are little understood conditions, especially in patients suffering from allergic respiratory disorders such as persistent rhinitis. Objective: The present study aims to evaluate the non-IgE-mediated immunoreactivity against common allergens, in allergen-reactive patients clinically diagnosed with persistent rhinitis without evidence of IgE-mediated hypersensitivity against these allergens. Methods: A group of 533 outpatients diagnosed with non-IgE-mediated allergen-reactive persistent rhinitis, were submitted to ex vivo Leukocyte Adherence Inhibition Tests (LAIT) with extracts of Dermatophagoides pteronyssinus, Hevea brasiliensis latex, dog dander, cat dander, cow’s milk proteins, beekeeping pollen and a mixture of airborne fungal extracts. Results: Cascade distribution column graphs were assembled according to the LAIT results among six ranges of Leukocyte Adherence Inhibition (LAI) of each allergen group. A column graph was plotted with the mean LAI results for each antigen for comparison. Conclusion: The LAIT is a potential tool to quantify the overall non-IgE-mediated cellular/humoral immunoreactivity against common allergens in patients clinically diagnosed with persistent rhinitis. Most studies must be done to evaluate the potential of the LAIT as a screening ex vivo provocation test to select the more reactive allergens to perform the diagnostic in vivo provocation tests.