Abstract
Western blot and enzyme-linked immunosorbent assay (ELISA) are antibody-mediated techniques which are widely used for the detection and characterization of alterations in host protein expression following H. pylori infection . Both techniques are highly specific and sensitive for protein detection, with Western blot detection sensitivity as low as picogram amounts of the protein of interest, while the typical ELISA detection range is 0.01-0.1ng. Here we provide an experimental example to demonstrate the application of these techniques for the determination of macrophage inflammatory responses following H. pylori infection .
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call