Abstract
<strong>ABSTRACT<em>. Background:</em></strong><em> </em>Growth factors used in health treatments can be obtained from a first-generation source called platelet-rich plasma. The variety of protocols to prepare PRP produces variable results regarding PRP activation time and its effects on cell proliferation and viability. <strong><em>Purpose:</em></strong><em> </em>To evaluate proliferation and cell viability of periodontal ligament fibroblasts and osteoblasts stimulated with PRP in several concentrations and times after PRP activation. <strong><em>Methods:</em></strong> An in vitro study was carried out using periodontal ligament fibroblast and osteoblast cell cultures. PRP from venous blood of a healthy adult was prepared through centrifugation and activated with 10 % CaCl<sub>2</sub>. The effect on cell proliferation after application of 1 %, 3 %, and 5% PRP and platelet-poor plasma was evaluated at 0, 12, 24, 48, and 72 hours after activation through MTS. The control group consisted of culture that did not receive any treatment. Data were analyzed using Chi square, Fisher, and McNemar tests. <strong><em>Results:</em></strong><em> </em>The cell viability assay showed statistically significant differences between the experimental and the control groups. Cell viability increased in cells treated with 5 % PRP 24 hours after activation (p=0.05). <strong><em>Conclusions:</em></strong><em> </em>Fibroblast and osteoblast cell lines tended to be more viable 24 hours after activation with 5% PRP.
Highlights
rowth factors used in health treatments can be obtained from a Arst-generation source called platelet-rich plasma
evaluate proliferation and cell viability of periodontal ligament Abroblasts and osteoblasts stimulated with plasma rico en plaquetas (PRP) in several concentrations and times aǼer PRP activation
study was carried out using periodontal ligament Abroblast
Summary
Para el cultivo celular se utilizaron líneas celulares de Abroblastos de ligamento periodontal (Clonetics® Human Fibroblast Cell System) y osteoblastos (Clonetics® Normal Human Osteoblast) adquiridas de la casa comercial Lonza. Los Abroblastos se adquirieron en tercer pase y se sembraron en medio de cultivo Stromal Cell Basal (Clonetics®) suplementado con suero fetal bovino (SFB) al 5%, insulina al 0,1 %, factor de crecimiento Abroblástico básico al 0,1% y gentamicina/anfotericina B al 0,1%. Los osteoblastos se adquirieron en segundo pase y se cultivaron en medio Normal Human Osteoblast Cell (Clonetics®) suplementado con SFB al 10%, ácido ascórbico al 0,1% y gentamicina/anfotericina B al 0,1%. Ambos cultivos se llevaron a cabo siguiendo el protocolo de la casa fabricante. Posteriormente, al alcanzar entre 70% y 80% de conÁuencia, en cuarto pase para los Abroblastos y quinto pase para los osteoblastos, los cultivos celulares se tripsinizaron
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