Evaluación de un antimicrobiano sobre la adhesión de Candida albicans a materiales de prótesis dentales

Abstract

Objective: to evaluate the effect of AlphaSan® on C. albicans adhesion to acrylic and polyamide resins. Methods: 2 x 2 mm squares were made with acrylic resin (AR) and polyamide resin (PR). Four study groups (n=12) were established: Group A: AR without AlphaSan®, Group B: AR with AlphaSan®, Group C: RP without AlphaSan®, Group D: RP with AlphaSan®. All groups were incubated in BHI broth with C. albicans ATCC at 36°C for 48 hours. Quantification of fungal adherence was performed by confocal microscopy (CM) and by means of an aqueous solution of crystal violet (CV), measuring the absorbance at 570 nm. Results: In the quantification of C. albicans adherence with CM, a 50% decrease (p =0.03) was observed between the treated groups (B and D) in relation to those not treated with AlphaSan® (A and C). No significant difference in C. albicans adherence was found between groups B and D (p > 0.5). C. albicans adherence, by CV, decreased by 55% (p= 0.01) in AR with AlphaSan® compared to AR without AlphaSan®. On the other hand, it was observed that fungal adherence decreased by 61% (p=0.01) in RP with AlphaSan® compared to RP without AlphaSan®. No significant difference was observed between the samples treated with the antifungal studied (p >0.05). Conclusion: Fungal adherence to the materials treated with AlphaSan® was limited to 50%, this would be due to the fact that the fungus presents a rigid cell wall (glucans and chitin), which provides a physical barrier for the penetration of different compounds. Consequently, it could be assumed that AlphaSan® would act on the cell wall, which would result in 50% of the fungi being able to adhere to the dental materials.