Evaluación de la microcirculación con sidestream dark field (SDF) Microscan® como método de monitorización en microcirugía en modelo porcino

Abstract

AimDetermine the feasibility of using SDF Microscan® as a non-invasive method for monitoring free flap microcirculation, and compare it to other methods. Materials and methodsExperimental study. In 8 pigs a pectoral myocutaneous flap was raised. Microcirculation was evaluated using: SDF Microscan®, near infrared spectroscopy (NIRS), clinical examination and Doppler. Venous, arterial and total occlusion was performed by clamping the vascular pedicle. Mean time to blood flow impairment diagnosis was measured. ResultsSDF in venous occlusion: reduced microcirculatory flow index at: 51s (59-62). SDF in arterial occlusion: reduced microcirculatory flow index at: 3s (1-5). SDF in total vascular occlusion: reduced microcirculatory flow index at: 3.5s (2-5). NIRS in venous occlusion: SatO2 decrease was 15.2±5.3%. NIRS in arterial occlusion: SatO2 decrease was 23.9±13.8%. NIRS in total vascular occlusion: SatO2 decrease was 23.85±13.9%. Doppler in venous occlusion: The signal did not disappear. Doppler arterial and total vascular occlusion disappears at 2s. The clinical changes were later than SDF. ConclusionMicrocirculation monitoring is feasible using SDF Microscan® in a pig model. This method allows to detect blood flow disruption earlier than NIRS and clinical evaluation.