Abstract
A simple sensitive spectrofluorimetric method was developed for the determination of oxytetracycline (OTC). Ethyl substituted β-cyclodextrin (DE- β-CD) can remarkably enhance the fluorescence intensity of the OTC-Eu 3+ complex at λ = 612 nm and the enhanced fluorescence intensity of Eu 3+ ion is in proportion to the concentration of OTC. Optimum conditions for the determination of OTC were also investigated. The linear range of this method for the determination of OTC is 8.28×10 −8–1.73×10 −5 mol/L and the limit of detection is 6.76×10 −9 mol/L, respectively. The developed method is practical and relatively free interference from coexisting substances and can be successfully applied to determination of OTC in samples of urine, serum, feed of chook and milk. The enhancement of the fluorescence at λ em=612 nm of Eu 3+ ion is because of the inclusion complex OTC-Eu 3+-DE- β-CD.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
