Abstract
Traditional, complementary, and integrative medicine are globally accepted alternative methods for the treatment of diabetes mellitus (DM). However, the mechanism of anti-diabetic effects of Helianthus tuberosus L. remains unproven. In the present study, antioxidant and anti-diabetic activity of the tubers of H. tuberosus were studied in detail. Methanolic extracts of H. tuberosus tubers were subjected to solvent fractionation method by increasing the polarity of the solvent using n-hexane, and ethyl acetate. The obtained methanol extracts and its fractions were subjected to free radical scavenging activity (DPPH and ABTS assay) and in vitro enzyme (α-amylase and α-glucosidase) inhibition assay. Moreover, glucose uptake in insulin-resistant HepG2 cell line was analyzed. The preliminary phytochemical analysis confirmed the presence of phenolic and flavonoid compounds in the active fraction. The radical scavenging and in vitro diabetic related enzyme inhibitory activities were found to be dose dependent. The maximum ABTS+ and DPPH scavenging activity was documented in ethyl acetate fraction of the H. tuberosus followed by methanol extract, hexane fraction, and methanol fraction. We also found that H. tuberosus showed a less toxicity in mouse fibroblast cells and enhance the glucose uptake in insulin-resistant HepG2 cells. Besides, the ethyl acetate fraction of the H. tuberosus analyzed by UPLC-QTOF-MS-MS and GC/MS revealed the presence of phenolic compounds such as neochlorogenic acid, chlorogenic acid, caffeic acid, 5-O-(4-coumaroyl)-quinic acid, feruloylquinic acid, caffeoylquinic acid, isoxazolidine, salicylic acid β-D-glucoside, dicaffeoylquinic acid isomers, salvianolic acid derivative isomers, and 1,4 dicaffeoylquinic acid etc. Among the identified phytochemicals, six were chosen for molecular docking study to explore their its inhibitory interactions with α-amylase and α-glucosidase. Taken together, the findings of the present study suggested that phytocompounds of EAF were responsible for the significant in vitro antioxidant, wound-healing, and anti-diabetic activities.
Highlights
The GS-MS analysis documented that several phytocompounds are present in the extracts and active fraction of the H. tuberosus, which are responsible for its antioxidant and anti-diabetic activity
The preliminary phytochemical studies revealed that the extract was enriched with phenolic and flavonoid compounds, which are responsible for their antioxidant activity
Α-amylase and α-glycosidase inhibition assays were performed to document the anti-diabetic index of the H. tuberosus methanolic extract and its active fractions
Summary
Diabetes drug market is valued of 57000 million USD in 2018 and it is expected to reach 87000 million USD by 2025 These syntactic drugs cause severe side effects. H. tuberosus tuber was one of the important ingredients in Korean soup [11] It is one of the unique plants that contain all the essential amino acids, which are the necessary nutrients for humans. Unauthorized folk medicine practitioners have reported that the plant material might have potential to ameliorate insulin sensitivity through decreasing the fasting glucose level in diabetic patients. Considering all these facts, the present study aims at the detailed scientific validation of antioxidant, wound-healing, and anti-diabetic effects of active organic fraction from H. tuberosus
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
