Screening of Aerial Parts of Kigelia Pinnata for its Antioxidant, Wound Healing and Antimicrobial Activity

Abstract

The antioxidant activity was performed by DPPH Scavenging activity method and Hydrogen peroxide scavenging activity method. Ascorbic acid was taken as standard in both methods. DPPH and Hydrogen peroxide scavenging activity methods of 100µg/ml extracts shows better % inhibition as compared to control and 50 µg/ml extracts. In DPPH Scavenging activity % inhibition of 100µg/ml methanolic extracts was 60.72% and in Hydrogen peroxide scavenging activity it was found to be 26%. Wound healing activity was performed by Incision wound model and Excision wound model. In incision wound healing model, the tensile strength of the 4% test group and the Neosporin ointment treated group were comparable to each other. Tensile strength of 2% extract ointment showed lesser 3.38 gm/mm2 but significant increase in tensile strength compared to the control group 2.91 gm/mm2. The tensile strength of 4% test group was found to be 3.44 gm/mm2. Thus both concentrations of the extract as well as the standard drug showed a significant increase in tensile strength on the 9th day. In excision models the animals treated with the 4% w/w KPME ointment were shown to healed completely as compared to 2%w/w which was also at the complete healing stage as compared to control treated and the standard drug (Neosporin) treated group. The epithelization period of standard group and treated group was less in comparison with that of simple ointment base treated groups. The percentage of wound contraction was much more with the 4% w/w extract was similar to that of Neosporin treated group. 2%w/w treated group of animals showed significant wound contraction. In antimicrobial activity, both antibacterial and antifungal potentiality was evaluated. In mic of antibacterial methanolic extract of Kigelia pinnta inhibit strains of E.coli and strain of Shigella boyelii, Salmonella aureus and Baccilus subtitis were found to be completely resistant. In mic of antifugal activity, all the fungal strain were inhibited by methanolic extract of Kigelia pinnata. These findings may provide a lead for further investigation of the overall pharmacological aciotns of Kigelia pinnata methanolic extract in more appropriate model.
 Keywords: Kigelia pinnata, DPPH, Anti-oxidant, DMSO, ANOVA