Abstract

Photochemical synthesis of ethanol from acetate was investigated with the system aldehyde (AldDH) and alcohol (ADH) dehydrogenases from Yeast, and methylviologen (MV2+) photoreduction by the visible light photosensitization of chlorophyll derivative, chlorin-e6 of zinc complex (ZnChl-e6) in the presence of NADPH as an electron donating reagent. Irradiation of a solution containing NADPH, ZnChl-e6, MV2+, AldDH, ADH and sodium acetate with visible light resulted in ethanol synthesis. The concentration of ethanol produced was 1.4mM after 150min irradiation under the condition of NADPH (3.3mM), ZnChl-e6 (100μM), MV2+ (12mM), AldDH (0.22μM), ADH (6.7nM) and sodium acetate (30mM) in 50mM of sodium pyrophosphate buffer pH 7.4).

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