Abstract

The potential of bioethanol generation using the mixture of waste French fries (WFF) and municipal wastewater (MWW) via separate hydrolysis and fermentation (SHF) was evaluated in this study. The effect of WFF substrate loading (SL, 10%, 16%, and 20%, w/v) on the SHF was also examined. Both glucose production and hydrolysis efficiency increased with increasing of SL from 10 to 16% and the maximum glucose yield of 0.236g glucose/g WFF and hydrolysis efficiency of 91.9% were obtained at SL of 16%. However, the glucose production and hydrolysis efficiency decreased when the SL further increased to 20% due to the inhibition on enzyme caused by higher glucose production. The mixture hydrolysate was then used as feedstock for ethanol fermentation. The maximum ethanol production of 22.69g/L was obtained from SL of 16%. The highest rate of glucose conversion to ethanol was 84.2%. The results demonstrated that the mixture of WFF and MWW could be used for ethanol production by the SHF.

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