Ethanol Extract with Black Cumin (Nigella Sativa) Against sFlt‑1 Level and VEGF Serum on Laboratory Mice with Preeclampsia

Abstract

Introduction: Preeclampsia is one of the complications that occur in pregnancies. This study was aimedto study the factors that affect the of giving ethanol extract with black cumin (Nigella sativa) against sFlt-1level and VEGF serum on laboratory mice induced preeclampsia.Method: Laboratory experimental research with posttest only control group design. This study used 30BALB/C laboratory mice, divided into 6 groups, namely negative controls: pregnant mice injected serumfrom normal pregnant women, positive controls; mice modeled preeclampsia, and treatment groups 1, 2,3 and 4 are preeclampsia mice received a dose of 500 mg, 1000 mg, 1500 mg and 2000 mg/kg weight ofNigella sativa ethanol extract for 5 days. Statistical analysis using ANOVAResult: The mean serum sFlt-1 level in mice modeled preeclampsia and treatment group dose 500mg,1000mg, 1500mg and 2000mg (2510.3+182.2 pg/mL, 2142.5+171.9 pg/mL, 1309+161.3 p/mL, and1500+169.9, respectively) pg/mL) showed a significant difference (p<0.05) and found a decrease in serumsFlt-1 levels with increasing doses. The mean serum VEGF levels in preeclampsia mice and treatment groupswere 500 mg, 1000 mg, 1500 mg and 2000 mg (50.25±2.85b pg/mL, 60.18±4.81c pg/mL respectively,71.89±2.38d pg/mL, 66.51±1.87 e pg/mL) showed a significant difference (p<0.05) and found an increase inserum VEGF levels as the dose increased.Conclusion: Giving of Black Cumin extract (Nigella sativa) decreases serum sFlt-1 levels and increasesserum VEGF levels in preeclampsia mice model and the effect is dependent dose.