Abstract
Background:Black sugarcane contains policosanol, quercetin antioxidant and 14-3-3 protein. These compounds are proven in a pre-clinical test of their ability to reduce brain ischemia. There are no pre-clinic test that prove the neuro-protectant act of black sugarcane extract on ischemia-induced rats, and how its mechanism to protect brain is not yet clear. This study’s purpose is to find the effect of ethanol extract of black sugarcane (BSEE) towards brains’ ischemic volume and to find is there any Bax role on the BSEE’s neuroprotectant mechanism.Methods: This study used fifteen male rats (Wistar). The rats were divided into three groups: group A that had been induced with BCCAO and received BSSE (group A), the rats that had been induced with BCCAO (group B), and sham group (group C). BSEE were given for a week, before bilateral ligation of common carotid artery. The BCCAO duration is 20 minutes, reperfusion periods of 2 hours. Rats’ head decapitation is done two hours after ligation. Brain tissues were colored with TTC. Ischemic brain area were analyzed using Image I software. Bax’ expression scoring was done in the brain preparations with immonohistochemical with Anti-Bax antibody. Anti-Bax proteins were anylized using ALLRED Score, while the statistic analyzing was done with One Way ANOVA.Results: There is a difference of ischemia volume’s mean (p=0,018) and bax protein expression (p=0,013) on the rats brain.Conclusion: BSEE is able to reduce ischemia volume and Bax protein expression on the rats’ brain.International Journal of Human and Health Sciences Vol. 04 No. 02 April’20 Page : 102-108
Highlights
Black sugarcane contains policosanol, quercetin antioxidant and 14-3-3 protein
The development of rat stroke model bilateral common carotid artery occlusion (BCCAO) is giving scientists the possibility to induce a preclinic study about any medications or herbs that might prevent or cure the ischemic brain, including stroke [1]
Previous studies had proven that boiled water from boiled black sugarcane can lower the ischemia volume of the rats’ brain post-BCCAO [4]
Summary
The development of rat stroke model bilateral common carotid artery occlusion (BCCAO) is giving scientists the possibility to induce a preclinic study about any medications or herbs that might prevent or cure the ischemic brain, including stroke [1]. The administration of 200 mg/ kgBBpolicosannol on stroke-induced Mongolian gerbils can lower the cerebral ischemic [3]. Previous studies had proven that boiled water from boiled black sugarcane can lower the ischemia volume of the rats’ brain post-BCCAO [4]. The antioxidant and protein in black sugarcane are able to rehabilitate ischemic brain. It is known that protein 14-3-3 in black sugarcane can regulate Bax expression in neuron degeneration 5). The previous study used water from boiled black sugarcane. The study related to Sugarcane Ethanol Extract’s administration effect on the Bax expression of the ischemic rats is not yet conducted. We need to induced a research on the effect of the black sugarcane policosanol extract on the Bax expression in the brain of PostBCCAO rats
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