Abstract
This study evaluates changes in the serum proteinogram and concentrations of copper, iron, and zinc in Santa Ines primiparous ewes with experimentally induced Staphylococcus aureus mastitis. The right mammary gland of 10 animals were inoculated with 1,0x104 UFC/mL. The clinical exam, poliacrilamide gel eletrophoresis (SDS-PAGE), and determination of Cu, Fe, Zn, and plasma fibrinogen (PF) levels were carried out just before inoculation and after 12, 24, 36, 48, 60, 72, 84, 96, 108, 120, 132, 168, 180, 288, and 336 hours. All animals presented mastitis and subsequent loss of function of the gland. A total of 23 protein fractions were detected. Their molecular weights (MW) varied between 26,000 and 185,000 daltons (Da), which allowed the identification of positive and negative acute phase proteins, immunoglobulins (IgA and IgG), and some nonidentified proteins. A positive correlation between PF and ceruloplasmin (r=0,74), haptoglobin (r=0,62) and IgA (r=0,62) was also evidenced. Results revealed the importance of ceruloplasmin and haptoglobin as acute phase proteins in intramammary infections of ewes and reiterate fibrinogen as an inflammatory marker. Serum alterations of Cu, Fe and Zn indicated inflammatory actions of mediators triggered by S. aureus.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
