Estudio de la estabilidad genética mediante el uso de marcadores RAPD en Ipomoea: una alternativa para la conservación de la diversidad biológica mediante técnicas moleculares

Abstract

This work was conducted in the Centre of Studies of Plant Biotechnology of the Faculty of Agricultural Sciences of the University of Granma. We used tuberous roots of the sweet potato clone INIVIT B 93-1 and vitroplants derived from somatic embryos coming from calli obtained from leaf tissue grown in MS medium. Tissue samples from both types of plant material were randomly obtained and their genetic stability analyzed by molecular analysis of the ADN (RAPD), using ten arbitrary primers (OPF y OPA) and evaluating the bands in terms of presence and absence. Such a molecular monitoring is required for any study of genetic diversity conservation to be conducted in different localities, regions and countries. As a result, seven primers produced an acceptable amplification, indicated by the intensity of the bands found in the donor and the somatic embryo-derived vitroplants. The high monomorphism appearing between both materials gives more accurate information for the establishment of strategies and procedures for the conservation and diffusion of the biodiversity.