蛍光測定法による尿中Estrogensの臨床的研究

Abstract

Urinary estrogens (E) were determined by means of fluorimetric method described previously in normal young adults and normal aged adults of both sex as well as in patients with pituitary-adrenocortical disorders, gonadal disease and chronic liver disease, totalling 80 subjects. Each fraction of estrone (EO), estradiol (ED) and estriol (ET)was measured in every subjects. In a part of them, in where ACTH-test with daily injections of 20 units ACTH-Z for 3 days, suppression test with Dexamethasone and Gonadotropin test were performed, change of urinary excretion on estrogens was also studied. The results obtained from thus measured 222 times totally are summerized as follows.1) In 8 subjects of normal young adult males(20-28 years old), the mean of daily excretion of urinary total estrogens (E) and its standard deviation (SD) were found to be 16.35×5.03μg(EO: 3.03×1.69μg, ED: 2.63×1.73μg and ET: 10.58×2.96μg). The mean and its SD of daily excretion per 1 m2 of body surface area, measured in 6out of 8 sublects, was 7.32×3.82μg. The means and their SD of total E in 7normal young menstruating femals (20~34 years old) during follicular phase and luteal phase were 23.07×12.2μg(EO: 5.97×3.10μg, ED: 4.31×2.08μg and ET: 13.21×4.39μg)and 41.55×14.40μg(EO: 12.73×2.50μg, ED: 7.89×3.40μg and ET: 21.00×5.45μg)respectively. The mean and the SD in 8 normal aged males(70-84years old)was 9.88×4.73μg for total E(EO: 1.51×0.80μg, ED: 1.77×1.35μg and ET: 6.60×3.47μg)with a conversion value per 1 m2 of body surface area as 7.32×3.82μg which was calculated in 5 out of 8 subjects. The means and their SD in 8 normal aged feamles(69-84 years old)was 8.45×3.12μg for total E(EO: 1.56×1.34μg, ED: 1.90×1.52μg and ET: 5.00×2.73μg)and 7.18×3.26μg for E excretion per 1 m2 of body surface area calculated in 5 out of 8 subjects.2) A change of total E excretion was observed on ACTH-test in 2 normal young males, which illustrated that the daily excretions orderly increased by injection of ACTH with the maximum value being on the third day and rapid return to the preinjected value after discontinuation of the injection. The minimum, maximum and average values of the sum of amount of increases on the second and the third days of ACTH injection were 15.1μg,116.1μg and 49.9μg respectively in normal adult males,31.4μg,96.6μg and 50.0μg espectively in 5 normal menstruating females,8.3μg 37.5μg and 24.6μg respectively in 5 normal aged males and 6.4μg,4.97μg and 19.1μg respectively in 4 normal aged females.