Abstract
Differential protein expression during germination as a result of a water deficit associated with variable osmotic pressure in snap-beans. Snap-beans (Phaseolus vulgaris L.) have a high nutritionally value. Successful seedling emergence is dependent on the availability of water and is considered the most critical phase in plant development. The objectives of this work were to simulate water deficiency at the beginning of the germination period. Snap-bean seeds of the variety 'Perola' were submitted to osmotic stress under laboratory conditions. Mannitol, CaCl2, MgCl2 and NaCl were used to develop five degrees of osmotic potential: 0, -0.3, -0.6, -0.9 and -1.2 MPa. Van't Hoff's equation was used to calculate the osmotic potential. The protein pattern of the total soluble proteins after electrophoresis was evaluated with SDS-PAGE. The response parameters were evaluated including the germination, the vigour classification, the root dry weight, the shoot dry weight and the resultant differential protein expression. The experimental design was completely randomised. Data were analysed using the F test (ANOVA), and polynomial regression was used for the osmotic potential levels for each parameter. The protein banding pattern was evaluated using gel imaging. The simulation of a water deficiency resulted in the perception of a stress and a subsequent response to NaCl in all of the parameters that were evaluated. The harsh effects of the NaCl were validated as the lack of the expression of low molecular weight proteins. Proteins with molecular weights of 110 and 30 kDa were indicative of water stress but not of salinity.
Highlights
Introdução O feijão é considerado uma cultura de ampla adaptação climática, importante para a nutrição da população brasileira e fonte de renda dos pequenos, médios e grandes produtores
O agente osmótico manitol causou reduções na germinação, no comprimento e massa seca de parte aérea e de raízes, e de plântulas normais de feijão em potenciais de -0,4 a -0,6 MPa
A expressão das proteínas diferenciadoras, algumas descritas como chaperonas, pode ter refletido na melhor resposta fisiológica observada nestes osmóticos, em relação ao CaCl2 e NaCl, como, por exemplo, na germinação (Figura 1A) e na massa total de plântulas (Figura 3A) no potencial mais restritivo ao crescimento, -1,2 MPa
Summary
Introdução O feijão é considerado uma cultura de ampla adaptação climática, importante para a nutrição da população brasileira e fonte de renda dos pequenos, médios e grandes produtores. À medida que a restrição hídrica aumentou, o vigor, avaliado por meio da classificação de vigor, foi reduzido (Figura 1B), sendo este mais afetado nos potenciais -0,9 e -1,2 MPa, principalmente no osmótico NaCl, corroborando Moraes e Meneses (2003) e Machado Neto et al (2004).
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