Abstract
The direct effects of estradiol-17β (E<sub>2</sub>) on phosphate (P<sub>i</sub>) uptake and on DNA synthesis in the primary rabbit kidney proximal tubule cells (PTCs) have been investigated. In the present study, E<sub>2</sub> (>10<sup>–9</sup>M, over 9 days) causes an increase both in [<sup>3</sup>H]thymidine incorporation and the number of PTCs. The anti-estrogen tamoxifen completely prevented the E<sub>2</sub>-induced increase in [<sup>3</sup>H]thymidine incorporation, and ameliorated the stimulatory effect of E<sub>2</sub> on growth. E<sub>2</sub> (>10<sup>–9 </sup>M, over 5 days) also stimulated the P<sub>i</sub> uptake and its effect was due to the V<sub>max</sub> values but not to the K<sub>m</sub> value for P<sub>i</sub> uptake. Estriol and estrone also exerted significant stimulatory effects on P<sub>i</sub> uptake. Progesterone, tamoxifen, actinomycin D and cycloheximide prevented the E<sub>2</sub>-induced stimulation of P<sub>i</sub> uptake. In conclusion, estrogens at physiological concentrations stimulate P<sub>i</sub> uptake and DNA synthesis in the renal proximal tubule cells, and these effects are estrogen receptor mediated.
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