Abstract
Cryopreservation is the main functional means for storage of excess embryos produced from artificial reproductive technologies; the process assumes embryos chemical nature is highly conserved across embryos of the same species. However, in practice there appears to be a high degree of variability in embryo tolerance to cryopreservation, suggesting potential differences in embryo chemistry. The objective of the current study was to develop reproducible means of estimating relative embryos weight and associating those weights to lipid content. Relative embryo weights of frozen/thawed in-vivo Jersey and Crossbred beef breed embryos were determined using a modified specific gravity chamber. Embryo weights were then correlated with lipid content. Results suggest that beef cattle embryos are 20% - 27% heavier than Jersey embryos (P
Highlights
The first successful cryopreservation of a preimplantation embryo was performed in 1972 when the embryo was cooled to −80 ̊C at 1 degree/minute and submerged in liquid nitrogen [1]
It has been suggested that the low conception rates in Jersey cattle are associated with embryo intracellular lipids, which have been linked to cell damage during cryopreservation [1] [4] [8] [9]
Low pregnancy rates are suggested to be associated with intracellular lipids of the embryo, which have been linked to cell damage during cryopreservation [1] [8]
Summary
The first successful cryopreservation of a preimplantation embryo was performed in 1972 when the embryo was cooled to −80 ̊C at 1 degree/minute and submerged in liquid nitrogen [1]. How to cite this paper: Weathers, J.D. and Prien, S.D. (2014) Estimation of Weight and Lipid Composition in Preimplantation Embryos from Jersey and Beef Breeds of Cattle. D. Prien main means of storing embryos and has become standard practice in reproductive industries, primarily human and bovine. Prien main means of storing embryos and has become standard practice in reproductive industries, primarily human and bovine While cattle breeds such as, Angus, Simmental, Herefords and others survive current cryopreservation techniques with conception rates approaching 60% to 70%, cryopreserved Jersey cattle embryos produce conceptions at a rate of approximately 20%, very similar to that of the human species [2] [3]
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