Abstract
The pH of vacuoles in tip cells of growing hyphae of a mycorrhizal fungus, Pisolithus tinctorius, was estimated by dual-wavelength microspectrofluorometry of 6-carboxyfluorescein. The vacuoles were found to have a modal pH of 7·0–7·5 and a pH range of 4·3–7·5. Vacuoles in penultimate cells tended to be more acid, with a modal group pH 5·5–6·0, although the range was similar at pH 4·8–7·2. The findings are discussed in relation to endocytosis and ageing of vacuoles in P. tinctorius.
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