Abstract
A rapid method for quantitating individual free fatty acids in plasma has been developed. An internal standard is added to plasma, the free fatty acids are extracted into an organic solvent, concentrated and injected into a gas chromatograph. For routine estimations, extraction and assay of a single sample takes about 30 min, and up to 30 samples can be assayed in a day. Seven free fatty acids (myristic, palmitic, palmitoleic, stearic, oleic, linoleic and linolenic acids) are routinely quantitated. This method has been used to monitor the therapy of patients on complete intravenous hyperalimentation and to study the effects of different substrates on the dog heart. It can also be used to investigate metabolic disorders involving fatty acids, e.g. Refsum's disease.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
