Estimation of Salt Tolerance Degree in some Selected Wheat Genotypes by Using Detection of Salt Tolerant Gene (TaSTK) and its Expression Under Salinity Conditions

Abstract

Molecular studies were carried out to detect salt tolerance gene (TaSTK) and estimate their expression in two selected cultivars (Dijilla and Furat) and one genotype (N3) of wheat under salinity condition (0, 15, 25 ds/m) as compared with salt sensitive cultivar (Tamooze-2). These cultivars and genotype were selected through plant breeding programs. The cDNA and gene amplification method used for this detection. Real-time PCR sybergreen method used to estimate the CT values and gene expression. The gene band length is 150 bp which appeared only in the selected materials (salt tolerance), while this gene absent in the salt sensitive culture (Tamooze-2). Amount and expression of TaSTK gene to be enhanced under salt conditions, and the degree of salt related enhancement was greatly only in salt-tolerant materials. Excess expression and amount of the TaSTK gene were at high salinity levels. At all salinity levels also the results showed that the amount and expression of this gene were proximately similar in all selected materials by contrast, there were no amount and expression of this gene in sensitive culture (local cultivar).