Abstract
High soil salinity is a major abiotic stress in plant production worldwide. <i>TaNIP</i> gene was identified and cloned through the gene chip expression analysis of a salt- tolerant wheat mutant RH8706-49 under salt stress. Quantitative reverse transcription – PCR (Q-RT-PCR) was used to detect <i>TaNIP</i> salt tolerant gene and its expression in some selected wheat genotype for salt tolerance through plant breeding programs. The results of qualitative PCR Reaction- cDNA and Quantitative Real-Time PCR showed that the gene band appeared only in the selected genotypes with length 189bp, while this band absent in salt sensitive cultivar (Iraq) under salinity and non-salinity condition. Amount and expression of <i>TaNIP</i> gene to be enhanced under salinity condition only in the selected salt tolerant genotype, and they increased with increasing salt level. Great expression and amount of <i>TaNIP</i> gene was at high salinity level (20 ds/m). The selected salt tolerant genotype had proximately similar amount and expression of <i>TaNIP</i> gene under all salinity condition, while there had no amounts and expression of this gene in sensitive cultivar (Iraq) therefore according to this gene (<i>TaNIP</i>) there is improvement realized in these selected genotypes for salt tolerance through plant breeding programs.
Highlights
Salinity is a big problem in the arid and semi-arid regions
The results revealed that the size of TaNIP gene amplification gene band was 189 bp according to the DNA ladder (Fig. 1)
The results indicated that TaNIP expression was induced by salts and this gene could be associated with the salt tolerance pathway in wheat
Summary
Salinity is a big problem in the arid and semi-arid regions. Soil salinity is a major abiotic stress severely affects agricultural productivity. Plants are affected throughout the growth process from germination to all others growth stages [1]. Induce new salt tolerant genotypes or cultivars are very important to overcome salinity problems and increase productivity of salt affected soils. Induce new germplasms with high salt tolerance requires new genetic sources of this character and more efficient techniques [2]. These germplasms are an attractive possibility for exploitation salt affected soils [3]
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