"Estimation of Saccharomyces cerevisiae Biomass Cultured in Cassava Mill Effluents"

Abstract

This study assessed the quantity of Saccharomyces cerevisiae biomass recovered from cassava mill effluents after 15 days of incubation. The Saccharomyces cerevisiae used in this study were isolated from palm wine, while the cassava mill effluents were obtained from rural community in Delta state, Nigeria. The raw effluents were filtered with double muslin cloth. Then after, it was heat treated before inoculating 10ml of S.cerevisiae inoculum into the 100ml cassava mill effluents in 250 flasks. The medium were shaked intermittently throughout the period of the study (15 days). Then after, the medium were carefully decanted and the resulted sludge was filtered using Whatman filter paper. The recovered biomass trapped in filtered paper was washed with distilled water and re-filtered. The recovered biomass was oven dried and weighed. The biomass recovered was 3.93±0.55g/L. The study showed that Saccharomyces cerevisiae biomass can be cultured in cassava mill effluents. Therefore, the use of Saccharomyces cerevisiae can be used to treat cassava mill effluents while generating biomass that can be utilized for other downstream application. Hence, there is the need for optimization of biomass produced through manipulation of the environmental parameters.