Amino Acid and Proximate Composition of <i>Saccharomyces cerevisiae</i> Biomass Cultivated in Cassava Mill Effluents

Abstract

Cassava mill effluent (CME) is generated during cassava processing into cassava flake or gari . The effluents affects the quality of the receiving environment such soil. It is could affect domestic animals such as goat and sheep when they ingest it, and retard normal growth and productivity of plants in the affected soil. This study evaluated amino acid and proximate composition of Saccharomyces cerevisiae cultivated in cassava mill effluents. S. cerevisiae used in this study was isolated from palm wine based on cultural, morphological, and physiological/biochemical characteristics. 10 ml of S. cerevisiae broth was inoculated into 100ml of sterile cassava mill effluents filtered with double muslin cloth. The medium was shaken intermittently between 7.00 to 19.00 hours’ time interval. The medium was decanted and subsequently filtered using Whatman filter paper. The resultant biomass was washed with distilled water and re-filtered. The sludge/biomass recovered were oven dried. Amino acid and proximate composition of the yeast biomass produced was analyzed using spectrophotometer. Proximate composition was also analyzed following standard procedure. Result showed that the total essential amino acid (40.88 g/100 g) was slightly higher than the Food and Agricultural Organization/World Health Organization (FAO/WHO) standard for feed use. While the total non-essential amino acid (21.56 g/100 g) was lower than FAO/WHO limits. Mean proximate composition was 17.01% (crude protein), 7.34% (ash), 56.40% (carbohydrate) and 407.13 kcal/100 g (calorific value/energy content). The findings of this study showed that during fermentation of cassava mill effluents with S. cerevisiae an appreciable amount protein is enriched considering the fact that the substrate is a waste.