Abstract
BackgroundPlants either in raw form or their isolated bioactive constituents are utilized as complementary and alternative medicine in various disorders. The present study was designed to evaluate chief phytochemical constituents of various fractions of Brachychiton populneus leaves and its antioxidative aptitude against free radicals.MethodsVarious fractions of B. populneus were prepared through solvent–solvent extraction technique based on their polarity and screened for phytochemical classes, total phenolic (TPC), flavonoid (TFC) and total tannin (TTC) content. Antioxidant effects of the extracts were manifested by in vitro multidimensional assays i.e. DPPH, hydroxyl radical scavenging, iron chelating, nitric oxide scavenging, β-carotene bleaching, phosphomolybdenum and reducing power assay.ResultsQualitative screening of various fractions of B. populneus ensured the presence of alkaloids, saponins, terpenoids, phenols, tannins, triterpenoids and flavonoids. Quantitative analysis revealed that aqueous fraction (BPA) showed maximum quantity of TPC and TFC followed by BPE and BPB. In terms of IC50 values BPA exhibited minimum values in all the in vitro antioxidant assays. However, the phytochemicals and yield did not accumulate in various fractions on polarity.ConclusionOur results indicated the presence of various polyphenolics, flavonoids, alkaloids etc. The yield of various fractions and qualitative phytochemical analysis did not correlate with polarity of solvents. Various antioxidant assays exhibited significant (p < 0.05) correlation with TPC and TFC and renders B. populneus with therapeutic potential against free-radical-associated oxidative damages and this effect was significant with BPA.
Highlights
Plants either in raw form or their isolated bioactive constituents are utilized as complementary and alternative medicine in various disorders
These results confirmed the presence of alkaloids, flavonoids, phenols, terpenoids, triterpenoids, quinones, oils and resins, phlobatannins, vitamin C, proteins and glycosides in all fractions of B. populneus
Anthraquinones were present in B. populneus methanol extract (BPM), B. populneus ethyl acetate fraction (BPE), B. populneus butanol fraction (BPB) and B. populneus aqueous fraction (BPA)
Summary
Plants either in raw form or their isolated bioactive constituents are utilized as complementary and alternative medicine in various disorders. The present study was designed to evaluate chief phytochemical constituents of various fractions of Brachychiton populneus leaves and its antioxidative aptitude against free radicals. Phytochemical studies are based on exploring plants for their use in the production of novel therapeutic drugs. Herbal medicinal plants synthesize vast range of secondary metabolites having therapeutic potential to cope with oxidative stress caused diseases [2]. The antioxidant activity of medicinal plants is primarily. The antioxidative aptitude of the therapeutic plants and their derived compounds is directly correlated with their strength to quench the reactive radicals by donation of electron; leading to radical chain reaction termination. Plants are a good source of dietary (i.e. exogenous) antioxidants. The curiosity in the exogenous plant antioxidants was first educed by the finding and consequent isolation of ascorbic acid from the plants [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
