Abstract
Simple, fast and reliable spectrophotometric methods were developed for determination of Ofloxacin in bulk and pharmaceutical dosage forms. The solutions of standard and the sample were prepared in Methanol. The quantitative determination of the drug was carried out using the second order Derivative Area under Curve method values measured at 295-301nm. Calibration graphs constructed at their wavelengths of determination were linear in the concentration range of Ofloxacin using 2-10?g/ml (r=0.9947) for second order Derivative Area under Curve spectrophotometric method. All the proposed methods have been extensively validated as per ICH guidelines. There was no significant difference between the performance of the proposed methods regarding the mean values and standard deviations. The developed methods were successfully applied to estimate the amount of Ofloxacin in pharmaceutical formulations.
Highlights
Ofloxacin is a synthetic broad spectrum antibacterial agent
Stock solution of 10μg/ml of Ofloxacin was prepared in Methanol, Second Order Derivative Area under Curve spectrophotometric analysis
The standard solutions were prepared by dilution of the stock solution with Methanol in a concentration range of 2, 04, 06, 08, and 10μg/ml with Methanol for Second Order Derivative Area under Curve spectrophotometric methods
Summary
Ofloxacin is a synthetic broad spectrum antibacterial agent. Ofloxacin[1] is a fluorinated carboxy-quinolone. It has official compendial applications in BP[2], USP[3], and EP[4]. The assay procedure mentioned in these pharmacopoeias uses non aqueous titration for estimation of ofloxacin. Literature survey reveals spectrophotometric methods, atomic absorption spectrometry, spectro-flurometry[5,6,7,8], HPLC[9]. Spectrophotometric method is reported an attempt has been made to develop new Zero Order and Area under Curve Spectrophotometric method for estimation of Ofloxacin in bulk and pharmaceutical formulations with good accuracy simplicity, precision and economy
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