ESTIMATION OF CEFEPIME HYDROCHLORIDE IN INJECTABLE DOSAGE FORM BY DIFFERENCE SPECTROPHOTOMETRY

Abstract

Cefepime is used as beta- lactam antibiotic. In present study, a simple, rapid, accurate, and economical difference-spectrophotometric method has been developed for the estimation of cefepime hydrochloride from pharmaceutical formulation. The developed method was statistically validated in accordance with ICH guideline on the basis of various parameters like linearity, accuracy, precision, sensitivity,and repeatability . The drug follows linearity in the concentration range 30-80 ?g/ml with a correlation coefficient value of 0.997 at ?max of 268.80 nm. The accuracy of the method was checked by recovery experiment performed at three different levels. The % recovery was found to be in the range of 99.30 to 100.25 %.The precision of the method was studied as an intraday; interday variations, and repeatability. The % RSD value < 2 indicates that the method is precise, reproducible and accurate. The proposed method was then successfully applied to pharmaceutical formulation. Amount of drug estimated was found between 99.7%, which was in good agreement with the label claim. Excipients used in injection did not interfere in the estimation of cefepime by developed method. The proposed method can be successfully employed in the routine analysis of drug in pharmaceutical dosage forms.