Abstract
A rapid, specific and accurate isocratic HPLC method was developed and validated for the assay of Amolodpine besylate and Metaprolol in pharmaceutical dosage forms. The assay involved an isocratic – elution of Amolodpine besylate and Metaprolol in ODS- C18 column using mobile phase composition consists of 70:30(v/v) of Methanol and 0.1M potassium di hydrogen ortho phosphate with 0.1% W/V 1-octane sulfonic acid with pH adjusted to 3.0.The wavelength of detection is 210 nm.The method showed good linearity in the range of 5.0-50.0 µg/mL for Amolodipine and 50 to 500 µg/mL for Metaprolol. The runtime of the method is 10 mins. The proposed method can be used for routine quality control samples in industry in bulk and in finished dosage forms. In present study, a rapid specific precise and validated HPLC method for the quantitative estimation of anolodpine and metaprolol in pharmaceutical dosage forms has been reported. The developed method can be applied to directly and easily to the analysis of the pharmaceutical tablet preparations. The percentage recoveries were near 100% for given methods. The method was completely validated and proven to be rugged. The excipients did not interfere in the analysis. The results showed that this method can be used for rapid determination of amolodipine and metaprolol in pharmaceutical tablet with precision, accuracy and specificity.
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