Abstract
A simple, precise, sensitive, fast and accurate high performance liquid chromatography method has been developed for the determination of atenolol using mixture of phosphate buffer and acetonitrile (53:47 v/v) as mobile phase. Buffer was prepared by mixing 0.02 M K2PO4and 0.003 M KH2PO4in equal proportion. Detection was carried out using UV detector at λmax230 nm. Column was ODS and dimensions of column was 25 mm × 4.6 mm. Atenolol was eluted out at retention time of 2.1 min. Method was validated at 1.2 mL/min flow rate. Calibration curve was linear between ranges of 40 to 200 mcg concentration. The limit of detection was calculates 120 nano gram and limit of quantitation is 510 nano gram. The relative standard deviation (RSD) of atenolol was 0.6. The percentage recovery of atenolol was 99.6%.
Highlights
Several works have been reported on atenolol determination in plasma, formulations adopting gas chromatographic techniques with an electron capture detector or HPLC, using reverse phase columns and UV or fluorometric detection[6,7,8,9,10]
Standard atenolol was dissolved in 15 mL of mobile phase and sonicated for 5 min
Reversed phase method was developed for estimation of atenolol
Summary
The mobile phase consisting of acetonitrile and phosphate buffer (47:53 v/v) was passed through 0.45 μ membrane filter and degas by altrasonications. Atenolol standard solution was prepared by separately dissolving 25 mg of pure drug in 25 mL Standard atenolol was dissolved in 15 mL of mobile phase and sonicated for 5 min. Diluted to 25 mL with mobile phase to get 100 μg mL-1 for atenolol and passed through 0.45 μm membrane filter.
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