Estimating Losses of Dry Matter from Simulated Rainfall on Bermudagrass and Orchardgrass Forages Using Cell Wall Components as Markers

Abstract

Previous methodologies to measure losses of dry matter (DM) in wilting hays subjected to natural or simulated rainfall have relied generally on gravimetric techniques, resulting in variable and questionable estimates of DM loss. The objective of this study was to evaluate the use of fiber components and acid detergent insoluble ash (ADIA) as internal plant markers for accurately predicting losses of DM in bermudagrass [Cynodon dactylon (L.) Pers.] and orchardgrass (Dactylis glomerata L.) forages that were damaged by simulated rainfall. For both forages, concentrations of neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose (HEMI), cellulose (CELL), lignin, and ADIA generally increased with the amount of simulated rainfall in primarily linear patterns. Recoveries of all internal markers were high (≥952 g kg−1) and were not affected by simulated rainfall for either forage (P ≥ 0.06). Predicted losses of DM increased in primarily linear patterns with simulated rainfall for both forages when concentrations of NDF, ADF, HEMI, CELL, and ADIA were used as internal markers. Linear regressions of predicted losses of DM on values determined gravimetrically were good (r2 ≥ 0.73; P ≤ 0.03) when concentrations of any fiber constituent or ADIA were used to calculate losses of DM; however, NDF was an especially effective internal marker (Y = 1.12X − 5; r2 = 0.97; P < 0.01).