Year-end Sale % OFF 
Abstract
Colon cancer is an abnormal growth of cells that occurs in the large intestine. Sometimes growth remains restricted for a relatively long time before it becomes a malignant tumor and then spreads through the intestinal wall to the lymph nodes and other parts of the body. The study aims to estimate the effectiveness and partial purification of lipoxygenase (LOX) enzyme and measure gamma-glutamyle transferase (GGT) activity in serum patients of colon cancer in Baghdad. The study included (80) case male patients with colon cancer with (50) samples of apparently healthy males (control) as comparison group. The result displayed a noteworthy increase in lipoxygenase effectiveness (805.0±517.23 IU/L) in serum of patients with colon cancer compared with control (114.6±49.77 IU/L). The enzyme was purified by the precipitation of the serum protein using (40% (NH4)2SO4) then removing the remaining salts by dialysis. The column of gel (sephadex G.100) was used to separate the enzyme from another protein, in this step a single peak was obtained. The effective part of lipoxygenase at yield (71.42%) and folds (11.033). The ion exchange chromatography (DEAE–CeA50) was used to isolate LOX isoenzyme, two bands (LOX1 and LOX2) were acquired with different degree of purity (16.372) and (12.16) folds respectively. The result displayed a noteworthy increase in the (GGT) activity in patients (58.69±16.94IU/L) (probability P≤ 0.000) compared with control (12.79±5.68 IU/L). The increase in activity of LOX can be used as a tumor marker to detect the colon cancer disease.
Highlights
Colorectal cancer is the fourth most common type of cancer in the world and the second most common cause of death, with (145.600) new case cancer diagnosis and (51.020) case deaths estimate in 2019(1)
It is an abnormal growth of cells that occurs in the large intestine, sometimes the growth remains restricted for a relatively long period before becoming a malignant tumor, it spreads through the bowel wall to the lymph glands and other parts of the body [2,3]
Colon cancer begins with noncancerous growth called, that later promotes on internal lining of the colon to grow slowly for 10-20 years [4]
Summary
Colorectal cancer is the fourth most common type of cancer in the world and the second most common cause of death, with (145.600) new case cancer diagnosis and (51.020) case deaths estimate in 2019(1). Colorectal cancer is treatable (especially by surgical removal), it leads to death if not detected early. It is an abnormal growth of cells that occurs in the large intestine, sometimes the growth remains restricted for a relatively long period before becoming a malignant tumor, it spreads through the bowel wall to the lymph glands and other parts of the body [2,3]. Colon cancer begins with noncancerous growth called (polyp), that later promotes on internal lining of the colon to grow slowly for 10-20 years [4]. The metabolism of fat in the human body, especially the pathway of arachidonic acid, plays a major role in chronic inflammation and colon cancer[6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
