Estimating dynamic changes of tissue attenuation coefficient during high-intensity focused ultrasound treatment

Abstract

Background This study investigated the dynamic changes of tissue attenuation coefficients before, during, and after high-intensity focused ultrasound (HIFU) treatment at different total acoustic powers (TAP) in ex vivo porcine muscle tissue. It further assessed the reliability of employing changes in tissue attenuation coefficient parameters as potential indicators of tissue thermal damage. Methods Two-dimensional pulse-echo radio frequency (RF) data were acquired before, during, and after HIFU exposure to estimate changes in least squares attenuation coefficient slope (Δβ) and attenuation coefficient intercept (Δα0). Using the acquired RF data, Δβ and Δα0 images, along with conventional B-mode ultrasound images, were constructed. The dynamic changes of Δβ and Δα0, averaged in the region of interest, were correlated with B-mode images obtained during the HIFU treatment process. Results At a HIFU exposure duration of 40 s and various HIFU intensities (737–1,068 W/cm2), Δβ and Δα0 increased rapidly to values in the ranges 1.5–2.5 dB/(MHz.cm) and 4–5 dB/cm, respectively. This rapid increase was accompanied with the appearance of bubble clouds in the B-mode images. Bubble activities appeared as strong hyperechoic regions in the B-mode images and caused fluctuations in the estimated Δβ and Δα0 values. After the treatment, Δβ and Δα0 values gradually decreased, accompanied by fade-out of hyperechoic spots in the B-mode images. At 10 min after the treatment, they reached values in ranges 0.75–1 dB/(MHz.cm) and 1–1.5 dB/cm, respectively, and remained stable within those ranges. At a long HIFU exposure duration of around 10 min and low HIFU intensity (117 W/cm2), Δβ and Δα0 gradually increased to values of 2.2 dB/(MHz.cm) and 2.2 dB/cm, respectively. This increase was not accompanied with the appearance of bubble clouds in the B-mode images. After HIFU treatment, Δβ and Δα0 gradually decreased to values of 1.8 dB/(MHz.cm) and 1.5 dB/cm, respectively, and remained stable at those values. Conclusions Δβ and Δα0 estimations were both potentially reliable indicators of tissue thermal damage. In addition, Δβ and Δα0 images both had significantly higher contrast-to-speckle ratios compared to the conventional B-mode images and outperformed the B-mode images in detecting HIFU thermal lesions at all investigated TAPs and exposure durations.