Abstract
ObjectsDysgeusia are caused by various factors such as chemotherapy, drugs, infection, and aging, and many people suffer. However, effective treatments for dysgeusia have not been established for drugs and foods. We have previously reported that the expression of lingual taste receptor T1R3 is reduced during chemotherapy induced dysgeusia. On the other hand, medaka, one kind of small fish, has many taste buds from mouth to gill, and the number is 5–6 times that of mice. In this study, we created a medaka that expressed the T1R3 gene with a fluorescent tag, and tried to visualize dysgeusia.MethodsWe established T1R3 knock‐in medaka using Crisper/cas9 system. The construct was microinjected into eggs immediately after medaka laying, and knock‐in was confirmed with a fluorescence microscope. It was confirmed that expression of T1R3 disappeared when reared in a solution containing an anticancer drug (5FU or platinum agent) using medaka of the F1 generation or later.ResultsWe succeeded in producing a medaka that visualized the taste receptors by microinjecting fluorescently labeled T1R3 into medaka eggs. The expression of T1R3 disappeared when reared in a solution containing an anticancer drug whereas several amino acids such as glutamate and overeating were effective in maintaining and increasing T1R3 expression.ConclusionsWe established screening for agents for improving dysgeusia using medaka.Support or Funding InformationThis study was supported by Japan Society of Promotion Science #18K11075 to RT.
Published Version
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