Abstract
Objective To establish the quality standard of Zhiqingshu lotion by high-performance liquid chromatography (HPLC). Methods HPLC was used to determine emodin, chrysophanol, caffeic acid, and berberine hydrochloride content, key water-soluble components of rhubarb, dandelion, and Phellodendron amurense in Zhiqingshu lotion. The macrophage inflammation model was used to analyze the anti-inflammatory effects of Zhiqingshu lotion. Results HPLC results showed that the contents of emodin, chrysophanol, caffeic acid, and berberine hydrochloride in Zhiqingshu lotion were 7.93 ± 2.25, 20.85 ± 4.27, 48.9 ± 6.79, and 58.4 ± 10.3 μg/mL, respectively. Moreover, RT-qPCR results showed that different concentrations of Zhiqingshu lotion significantly reduced the expression of inflammatory factor tumor necrosis factor- (TNF-) α and interleukin- (IL-) 1β in lipopolysaccharide-induced macrophages. Conclusion HPLC could quantitatively and qualitatively analyze and identify the main components of Zhiqingshu lotion as rhodopsin, rhodopsin, caffeic acid, and berberine hydrochloride. And Zhiqingshu lotion has an excellent anti-inflammatory effect. This method was simple and reliable and could be used for the identification of the ingredients and content of Zhiqingshu lotion, thus improving the quality control of the drug.
Highlights
Zhiqingshu lotion (ZQSL) is a traditional Chinese medicine compound formula developed based on the theory of traditional Chinese medicine
The methodological investigation of the high-performance liquid chromatography (HPLC) method was carried out, and the results showed that emodin (Y = 39748x + 5398:4, r = 0:9999), chrysophanol (Y = 51553x + 13753, r = 0:9999), caffeic acid mV
The precision test results showed that the relative standard deviation (RSD) of emodin, chrysophanol, caffeic acid, and berberine hydrochloride was 0.13%, 0.31%, 0.42%, and 0.32% (n = 5), respectively, indicating that the instrument had good precision
Summary
Zhiqingshu lotion (ZQSL) is a traditional Chinese medicine compound formula developed based on the theory of traditional Chinese medicine. The quality of traditional Chinese medicine compound prescriptions is essential in clinical treatment. It is extremely important to strengthen the quality control and work implementation of traditional Chinese medicine preparations, improve the safety of the clinical medication, and ensure the reliability of the efficacy [5]. The reference source for the quality standard specification for ZQSL is the Guangdong Province Preparation Registration Standard of Medical Institutions (Guangdong ZB20111771). This standard does not establish a content determination standard, only involving the pharmaceutical properties of the drug and the thin-layer chromatography identification of the main drug rhubarb [1, 6]. There are still big restrictions on the quality control and pharmaceutical efficacy evaluation of ZQSL
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