Establishment of peroxidation damage model of healthy human hepatic cells and the protective effect of salvianolic acid A

Abstract

Objective To select the appropriate concentration of hydrogen peroxide for the establishment of cell peroxidation damage model,and the applicable concentration and time of salvianolic acid A for protection.Methods The L02 ceils were divided into 3 groups.In group 1,L02 cells were subjected to hydrogen peroxide for 24 h with different concentrations.In group 2,L02 cells were treated with different concentrations of salvianolic acid A.In group 3,hydrogen peroxide was used to deal with L02 cells pretreated by salvianolic acid A with different concentrations for 24 h.Methyl thiazolyl tetrazolium (MTT) assay was used to measure the growth of L02 cells under a microscope.Results The inhibition ratio of growth of L02 cells treated with 160 μmol/L H2O2 was 35％,and part of the cells started to float and sporadic cell debris appeared.In group 2,the mean optical density was (1.704 3 ± 0.008 1) at 24 h in 20 nmol/L group,which was the highest,and significantly higher than in control group (1.544 3 ± 0.008 3,P ＜ 0.01).The mean optical density in group 3 was (1.565 0 ±0.041 6),which showed no significant difference from control groups (1.570 0-± 0.021 2,P ＞ 0.05).The mean optical density in the remaining groups at each time point was all below the control groups.Conclusion 160 μmol/L was the most suitable concentration for H2O2 to establish the cell peroxidation damage model.20 nmol/L salvianolic acid A could not only accelerate cell proliferation,but also alleviate the damage caused by hydrogen peroxide. Key words: Peroxidation injury; Salvianolic acid A; Liver disease