Effect of salvianolic acid B on mesenchymal stem cells proliferation and VEGF expression

Abstract

Objective To isolate,culture and purify rat bone marrow mesenchymal stem cells (MSCs) and observe the effect of salvianolic acid B on MSCs proliferation and vascular endothelial growth factor (VEGF) expression in vitro. Methods MSCs were isolated,cultured and purified in vitro by adherent method and identified by immunofluorescence detection of cell markers CD44 and CD34. MSCs were cultured in five groups of lower glucose DMEM media containing different concentrations of salvianolic acid B. The growth ability of cells was assayed with methyl thiazolyl tetrazolium(MTT) method. The expression of VEGF mRNA in the MSCs harvested at following 24 h,48 h and 72 h were analyzed with reverse transcription-polymerase chain reaction. Results Pure MSCs were obtained. The cultured cells marked with CD44 were positive and those with CD34 were negative by immunofluorescence detection,so it could be identified as MSCs. The cell proliferation was promoted by 0.3 mg/L and 3 mg/L salvianolic acid B as compared with control group (P<0.01,P<0.05),while 0.03 mg/L and 30 mg/L salvianolic acid B did not affect the proliferation of MSCs. The expression of VEGF mRNA of MSCs treated with salvianolic acid B at concentrations of 0.03,0.3,3 and 30 mg/L were increased significantly as compared with control group (P<0.05). Conclusion Pure MSCs can be obtained by adherent method and salvianolic acid B can promote the proliferation and the expression of VEGF mRNA of MSCs. Key words: Bone marrow; Mesenchymal stem cells; Cellculture; Salvianolic acid B; Vascular endothelial growth factor